干细胞研究
使用无创成像
实时监测干细胞培养
干细胞来源，如间充质干细胞、诱导多能干细胞和神经干细胞，正在被广泛地研究以作治疗使用。
培养干细胞并不简单，但它们在对抗难以治疗或根本无法治疗的病理方面具有巨大的潜力。
干细胞培养是一个长期的过程。 从隔离到扩张再到收获，这个过程可能需要数周时间。 许多细胞实验室都有自己的优化方案，旨在保持干细胞和干细胞增殖。 然而，批次间的差异会对最终产品的质量产生重大影响。
无创成像和延时成像可以为干细胞培养的监测和优化提供直接的解决方案。 成像方式可以让研究人员深入了解和控制他们的干细胞培养。
从缺氧室内成像干细胞
无需将细胞从培养箱中取出，使用云连接进行远程进行活细胞显微成像观察
间充质、造血、神经和胚胎干细胞的生理生态位提供 1-8% 的氧气。 这与通常在孵化器中维持的 21% 有显着差异。 缺氧室可以帮助确保理想的干细胞活力、产量和干细胞。 通过云计算使用远程连接对细胞培养进行成像，例如 使用 Lux2 可以帮助在获取微观数据时防止环境冲击。
增殖的多井分析
自动延时成像和汇合度检测
对于高通量分析和细胞系优化，获得动力学信息有很大的优势。 CytoSMART Omni 能够自动创建多孔板中整个孔的延时视频。 细胞培养生长率、形态变化和凋亡事件可以被可视化，然后使用自动图像分析进行分析。
CytoSMART Omni 是进行动力学分析以绘制细胞增殖图的理想选择，我们将其用作了解胰腺癌细胞系耐药性的工具。
Dr. Martin Sprick | 高级组长
海德堡干细胞技术与实验医学研究所
通过自动监控优化产量
自动汇合度监测支持
干细胞漫长培养过程需要耐心和仔细监测。 延时成像有助于跟踪培养融合和形态学发生的任何变化。 特别是对于 iPSC，重要的是集落不会过度生长，因为这会让干细胞不稳定。 CytoSMART Lux 2 Duo Kit 可以帮助跟踪培养箱内的细胞培养，并采用自动融合检测来密切且明确地监测培养。 CytoSMART Omni 可以扫描完整的烧瓶表面，例如 T-175 或 HYPERflasks，并用于检查培养的均匀性。
活细胞的无标记成像
干细胞具有明显的形态特征和生长模式。 能够探索和量化这些对培养优化和检测开发非常有益。群落检测 和 划痕愈合分析 受益于全孔分析的标准检测方法。 对于追踪比较单细胞，我们推荐使用 CytoSMART Lux 2 Duo Kit.
Technical specifications
Videos
Appnotes
Cell Motility Video Monitoring - Lux2
Cell Cytoxicity Assay to Analyze Drug Response
Cell Culture Monitoring - Lux2
Cell Motility Video Monitoring - Lux2
Movement of cells plays a critical role in the development of cancer. Analyzing the motility of cells in vitro is therefor important for many cancer researchers.
Live-cell imaging, and in particular label-free live-cell imaging, is well suited to capture dynamic processes in cell culture without potential side-effects of markers or dyes on the cells. In the text below we will discuss the suitability of the CytoSMARTTM
Lux for several assays relevant for cancer research
We will discuss
- Introduction to the Lux cell monitoring system
- Tube formation assays
- 2D migration assays
- 3D invasion assays
Cell Cytoxicity Assay to Analyze Drug Response
The effect of drugs and drug candidates on the viability of cells in culture can be determined using cell counting, live/dead assays and metabolic assays. However, these assays are often end-point measurements. Alternatively, cells can be monitored using bright-field microscopy, by creating time-lapse videos for a culture period of multiple days. To study the lasting effect of the drug candidate.
In this study the cytotoxic effect of Paclitaxel, a chemotherapy drug, was investigated for a range of concentrations. The effect on cell viability between drug concentrations was compared by analyzing confluency measurements obtained using automated live-cell imaging. The entire experiment was performed inside a CO2-incubator, ensuring optimal culturing conditions and cells were imaged every hour for a period of 3 days.
Cell Culture Monitoring - Lux2
Setting up cell cultures is easy enough. However monitoring your cultures and optimization is time-consuming and cumbersome. Waiting for the ideal confluency, quickly studying effects of various media means taking your cells in and out of the incubator more often than you would like.
Visualizing cell cultures from inside an incubator using a compact microscope that facilitates live cell imaging can overcome these issues. While live cell imaging has been restricted to costly, high-end devices, the CytoSMART Lux2 offers an affordable and easy-to-use alternative for virtually any lab. The CytoSMART Lux2 can be set up in minutes, enabling untrained users to quickly perform their own time-lapse recordings.
Images and videos can be easily accessed and retrieved from the CytoSMART cloud portal. Advanced functions, such as reporting of cell confluency, cell migration analysis and the option to use automatic confluency email alerts, can be applied to inform the user when certain culture conditions are reached (for example, once the cell culture has reached the desired confluency). Hence, the CytoSMART Lux2 can be used in many different ways to facilitate cell culture work and research.
In the following appnote several examples of applications of the CytoSMART Lux2 will be shown.
- Culturing cells in hypoxic conditions
- Standardizing cell culturing conditions
- The effect of confluency on transfection efficiency