Application details

Cell Confluence

Confluency analysis to monitor growth and proliferation

Cell confluence is defined as the percentage of the surface area of a 2D culture that is covered with cells. Commonly confluence assessment is used to determine when cells need to be passaged. Properly timing this moment is essential to maintain cell phenotype and culture quality.

Growth rate monitoring - video example CytoSMART Lux2

Follow the progression of your culture. The video below depicts the growth of C6 cells 120 hours after seeding. The accompanying graph is produced by the confluence detection algorithm in the CytoSMART cloud.

The cell coverage is plotted along the y-axis over the 120 hours culture period. The cell culture progresses from the lagging, to the exponential growth phase, until at nearly a hundred percent coverage a plateau is reached and growth is halted.

Cell area coverage - video example CytoSMART Lux2

See the migration of 3T3 cells over a period of 14 hours at a 15 min imaging interval. Cells are highlighted with a digital mask indicating detection by the CytoSMART confluence algorithm.

whole well analysis - application example CytoSMART Omni

Confluency measurements provide an overview on how cells are distributed throughout sample wells. In the figure bellow JnHEK cells were monitored over a period of 48 hours. Two features are highlighted. The CytoSMART Omni's ability to create bright-field images of large surfaces, while maintaining a detailed view of the cell population. Secondly integrated image analysis algorithms were used to determine confluency values at each time point. The bottom row of images display the confluency mask that is generated after analysis.

The graph displays the confluency in [%] of the top row in the example figure. Imaging and analysis was performed every two hours.

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